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Guide RNA Finder

Design optimal guide RNAs for CRISPR diagnostics in four steps.

Cas12 (DNA)

CRISPR-Cas12a nuclease

DNA targetingPAM dependent18–24 nt spacers

For DNA-based diagnostics. Requires a PAM sequence (TTTN/TTTV) upstream of the target. Collateral ssDNA cleavage enables lateral flow or fluorescence readout.

Activity prediction · optimal 21 nt

DR: AsCas12a (19 nt), LbCas12a (20 nt)

Cas13 (RNA)

CRISPR-Cas13a nuclease

RNA targetingPAM independent20–28 nt spacers

For RNA-based diagnostics. No PAM requirement — targets any RNA sequence. Collateral ssRNA cleavage enables fluorescence-based detection.

Activity prediction · optimal 28 nt

DR: LwaCas13a (35 nt), LwaCas13a opt (29 nt)

ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT

Open-source CRISPR guide RNA design and scoring for Cas12 and Cas13 diagnostic systems.

Resources

Finder Documentation Changelog Contact GitHub

Developed at

Fundacao Oswaldo Cruz - Parana

Instituto Carlos Chagas

hwalflorGitHub