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Guide RNA Finder

Design optimal guide RNAs for CRISPR diagnostics in four steps.

Cas12a (Cpf1)

DNA targeting · PAM dependent

18–24 nt spacers · collateral ssDNA cleavage

PAM sequence

Cas13a (C2c2)

RNA targeting · PAM independent

20–28 nt spacers · collateral ssRNA cleavage

ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT ATCG GCTA TACG CGAT ATCG TAGC GCTA ATCG TACG CGAT

Open-source CRISPR guide RNA design and scoring for Cas12 and Cas13 diagnostic systems.

Resources

Finder Documentation Changelog Contact GitHub

Developed at

Fundacao Oswaldo Cruz - Parana

Instituto Carlos Chagas

hwalflorGitHub