Changelog

0.1.0 — 2026-03-03

Initial public release of SPACER Web — a full-featured interface for CRISPR guide RNA design powered by the spacer-lib Rust engine.

Added

Guide RNA Finder with 4-step wizard — sequence input, enzyme/PAM configuration, optional features, and results
Multi-enzyme support for Cas12 (DNA-targeting, PAM-dependent) and Cas13 (RNA-targeting, PAM-independent)
Composite scoring engine (0–100) combining GC content, homopolymer, poly-T, activity prediction, and structure analysis
Tier classification system — Excellent (80–100), Good (60–79), Fair (40–59), Poor (0–39)
Quality flag analysis with detailed per-guide explanations of score deductions
ML activity prediction via EasyDesign (Cas12) and ADAPT (Cas13) models
RNA secondary structure scoring via Vienna RNA predictor
Assay score system — 9-component weighted scoring replacing simple composite, with per-guide score breakdowns and quality flag analysis
Variant analysis — multi-target scoring across MSA alignments, per-variant activity prediction, signal ratio filtering, and coverage statistics
BADGERS evolutionary spacer optimizer — multi-target detection and variant identification modes with fitness-based spacer design
Genome screening — off-target specificity analysis against reference genomes with mmap index support
Cas13 variant support — LwaCas13a, LbuCas13a, and PsmCas13b enzyme configurations
Primer design clustering strategies — PositionBased, ScorePriority, and MaximizeGoodCoverage algorithms with structure filtering via ntthal
MAFFT auto-alignment for unaligned MSA input sequences
RPA primer design for amplification workflows
Multi-format export — CSV, TSV, JSON, and FASTA with enzyme-aware nucleic acid format
NCBI sequence search and personal sequence library
Job system with real-time progress tracking and status management
Statistics dashboard with score distribution, GC content, and activity histograms
Service health monitoring dashboard
30+ page documentation site with full-text search
Authentication system via Better Auth (email/password, Google OAuth)
User feedback submission system with email notifications
AI assistant for CRISPR guide design questions
Responsive design for mobile and desktop
Dark mode support via next-themes

0.1.0 — 2026-03-03

Initial public release of SPACER Web — a full-featured interface for CRISPR guide RNA design powered by the spacer-lib Rust engine.

Added

Guide RNA Finder with 4-step wizard — sequence input, enzyme/PAM configuration, optional features, and results

Multi-enzyme support for Cas12 (DNA-targeting, PAM-dependent) and Cas13 (RNA-targeting, PAM-independent)

Composite scoring engine (0–100) combining GC content, homopolymer, poly-T, activity prediction, and structure analysis

Tier classification system — Excellent (80–100), Good (60–79), Fair (40–59), Poor (0–39)

Quality flag analysis with detailed per-guide explanations of score deductions

ML activity prediction via EasyDesign (Cas12) and ADAPT (Cas13) models

RNA secondary structure scoring via Vienna RNA predictor

Assay score system — 9-component weighted scoring replacing simple composite, with per-guide score breakdowns and quality flag analysis

Variant analysis — multi-target scoring across MSA alignments, per-variant activity prediction, signal ratio filtering, and coverage statistics

BADGERS evolutionary spacer optimizer — multi-target detection and variant identification modes with fitness-based spacer design

Genome screening — off-target specificity analysis against reference genomes with mmap index support

Cas13 variant support — LwaCas13a, LbuCas13a, and PsmCas13b enzyme configurations

Primer design clustering strategies — PositionBased, ScorePriority, and MaximizeGoodCoverage algorithms with structure filtering via ntthal

MAFFT auto-alignment for unaligned MSA input sequences

RPA primer design for amplification workflows

Multi-format export — CSV, TSV, JSON, and FASTA with enzyme-aware nucleic acid format

NCBI sequence search and personal sequence library

Job system with real-time progress tracking and status management

Statistics dashboard with score distribution, GC content, and activity histograms

Service health monitoring dashboard

30+ page documentation site with full-text search

Authentication system via Better Auth (email/password, Google OAuth)

User feedback submission system with email notifications

AI assistant for CRISPR guide design questions

Responsive design for mobile and desktop

Dark mode support via next-themes